diff --git a/tests/integration/test_config_substitutions/test.py b/tests/integration/test_config_substitutions/test.py
index 692b36f1fae..d2a7a4a7ae4 100644
--- a/tests/integration/test_config_substitutions/test.py
+++ b/tests/integration/test_config_substitutions/test.py
@@ -30,6 +30,12 @@ node6 = cluster.add_instance(
     },
     main_configs=["configs/include_from_source.xml"],
 )
+node7 = cluster.add_instance(
+    "node7",
+    user_configs=["configs/000-config_with_env_subst.xml", "configs/010-env_subst_override.xml"],
+    env_variables={"MAX_QUERY_SIZE": "121212"},
+    instance_env_variables=True,
+) # overridden with 424242
 
 
 @pytest.fixture(scope="module")
@@ -78,6 +84,10 @@ def test_config(start_cluster):
         node6.query("select value from system.settings where name = 'max_query_size'")
         == "99999\n"
     )
+    assert (
+        node7.query("select value from system.settings where name = 'max_query_size'")
+        == "424242\n"
+    )
 
 
 def test_include_config(start_cluster):